Test Selection

Specific test information including sample requirements, reference ranges, special requirements and contact details are available in our Tests Table

Sample Volumes and Collection Bottle Types

Sample Types

For details regarding sample volumes and specific collection bottle types click here.


Adding Tests to Existing Samples

Blood Sciences do not recommend or encourage the use of ‘add-on tests’. However, under specific circumstances additional tests may be added to existing requests provided the sample is viable and a valid request form is received To request additional tests on Pathology Samples in Biochemistry and Haematology, please request these electronically on ICE where possible. If you are unable to request additional tests on ICE, please use the Add on Requesting Form and hand deliver, scan or electronically complete the request form and send the completed form via email to bdgh-tr.barnsleypathologyreception@nhs.net . Urgent request may be telephoned to the laboratory but must be accompanied by a viable request form.Click here for further information.


Referral Laboratory Contact Details

Click here for addresses and telephone numbers of test referral laboratories listed in the test repertoire table.

Reference ranges


Reference ranges have been derived from various recognised sources and guidelines.

“Practical Haematology” 2006, Tenth Edition, SM Lewis, B J Bain, L Bates.

“Diagnosis in Paediatric Haematology”, 1996, Harry Smith.

“Blood Cells-A Practical Guide”, 2002, Third Edition, Barbara J Bain.

“Reference Intervals for a complete blood count determined on different automated haematology analysers”, Clin Chem Lab Med 2002;40:69-73. Jan Van Bossche et al

“Assessing the Bayer ADVIA 2120 Hematology Systems” Laboratory Hematology,  2005, Vol 11, Number 1, Neil Harris et al

“Total and Differential Leucocyte Counts in Infants”, Department of Paediatric Haematology, Sheffield Children’s Hospital, G J Bellamy et al

“Normal ranges from Sheffield Children’s Hospital” 2009


Reference ranges have been derived from various recognised sources and guidelines. The majority of reference ranges are aligned to the:

  • Pathology Harmony Group, Clinical Biochemistry Outcomes, January 2011 (http://www.acb.org.uk/docs/default-source/committees/scientific/guidelines/acb/pathology-harmony-ii-biochemistry.pdf?sfvrsn=2)

Additional ranges have been sourced from:

  • Annals of Clinical Biochemistry 1994 31 1-11
  • Annals of Clinical Biochemistry 2002 39:577-582
  • Annals of Clinical Biochemistry 2004 41: 17-38 JCEM 1996 81: 1378-1384
  • Annals of Clinical Biochemistry 2004 41:17-38 JCEM 1996 81:1378-1348
  • CALIPER Clinical Biochemistry 2014 47:812-815
  • Clinical Chemistry 190:36;344-
  • Clinical Chemistry 1988 34(8) 1625 – 1628
  • Clinical Chemistry Lab Med 2002; 40;1040-
  • European Journal Clinical Biochemistry 1997; 35:533-537
  • https://cks.nice.org.uk/vitamin-d-deficiency-in-adults-treatment-and-prevention#!scenario
  • http://www.sign.ac.uk/assets/sign124.pdf interpretation not RR
  • Information For Users Reference Range
  • JCEM 2017 102;1161 –
  • Journal Clinical Endocrinology Metabolism 2008 93;1526-
  • Journal Paedatrics 1997 131:252-
  • Leeds Steroids extraction assay
  • NICE ng28 updated May 2017
  • Protein Reference Unit Data, 2007
  • Soldin AACC paediatric ref ranges 6th ed 2007
  • Tietz N W Clin. guide to laboratory tests 4th edn. W.B. Saunders 2006
  • Werfen GEM 4000 user guide
  • W. Lee Clinical Chemistry (1997) 43, 154 – 157


For further information please contact the Clinical Biochemists