Collection of samples

 Nucleic Acid Amplification Test ( NAAT) – Patient self take instructions (Testing is no longer done for Chlamydia only)

The laboratory issues specific collection kits for Chlamydia/GC  testing using the Aptima Hologic Panther.

The swab to use is an orange Aptima multitest swab specimen collection kit for patient-collected specimens.

Urine ( male and female) should be collected using the yellow urine Aptima collection kits.

General Instructions for all collection methods:

Wash hands before starting

In the privacy of the examination room or toilet, undress as necessary, comfortably position yourself to maintain balance during the collection procedure.

Open the kit package, remove the swab and tube.

Warning : If at any time the contents of the tube are spilled on your skin, wash the affected area with soap and water. If the contents are splashed into your eyes, immediately flush your eyes with water. Notify the clinic/nurse/GP if irritation develops.

If the contents of the tube are spilled, request a new multitest swab kit.

DO NOT TAKE THE CONTENTS OF THE TUBE INTERNALLY

Vaginal swab specimen collection

If you are pregnant ensure you inform your healthcare provider.

Partially peel open the swab package and remove the swab – do not touch the soft swab tip or lay the swab down on a  surface

Hold the swab in your hand, placing your thumb and forefinger in the middle of the swab covering the score line ( black line)

Carefully insert the swab into the vagina about 2 inches ( 5 cm) inside the opening, gently rotate the swab for about 10 to 30 seconds, making sure the swab touches the side of the vagina, remove the swab.

While holding the swab, unscrew the top of the tube and place the swab into the liquid in the tube so that the score ( black) line is at the top of the tube.

Carefully break off the swab at the score line by pressing the shaft of the swab against the side of the tube and discard the top part of the swab shaft.

Screw the cap back onto the tube and ensure it is tightly fastened

Do not pierce the foil lid.

Ensure your name and date of birth is on the tube, hand back to the nurse.

Penile metal swab specimen collection

Partially peel open the swab package and remove the swab – do not touch the soft swab tip or lay the swab down on a  surface

Hold the swab in your hand, placing your thumb and forefinger in the middle of the swab covering the score line ( black line)

Uncircumcised men will have to roll the foreskin down before starting collection

Hold the penis with your free hand (the hand not holding the swab), using your other hand (with the swab), roll the swab at the tip or outside the opening to the penis through which you pass urine. Be sure to roll the swab completely around the opening to ensure you get a good sample. It is NOT necessary to insert the swab deep inside the penis

While holding the swab, unscrew the top of the tube and place the swab into the liquid in the tube so that the score ( black) line is at the top of the tube.

Carefully break off the swab at the score line by pressing the shaft of the swab against the side of the tube and discard the top part of the swab shaft.

Screw the cap back onto the tube and ensure it is tightly fastened

Ensure your name and date of birth is on the tube, hand back to the nurse.

Rectal swab

Partially peel open the swab package and remove the swab – do not touch the soft swab tip or lay the swab down on a  surface

Hold the swab in your hand, placing your thumb and forefinger in the middle of the swab covering the score line ( black line)

Carefully insert the swab into your rectum about 1-2 inches ( 3-5 cm) past the anal margin ( outside of the anus), and gently rotate for 5-10 seconds, withdraw the swab without touching your skin.

While holding the swab, unscrew the top of the tube and place the swab into the liquid in the tube so that the score ( black) line is at the top of the tube.

Carefully break off the swab at the score line by pressing the shaft of the swab against the side of the tube and discard the top part of the swab shaft.

Screw the cap back onto the tube and ensure it is tightly fastened

Do not pierce the foil lid.

Ensure your name and date of birth is on the tube, hand back to the nurse.

Throat swab

Partially peel open the swab package and remove the swab – do not touch the soft swab tip or lay the swab down on a  surface

Hold the swab in your hand, placing your thumb and forefinger in the middle of the swab covering the score line ( black line)

carefully insert the swab into your mouth, ensuring contact with both of your tonsils and the back of the throat, then withdraw the swab without touching the inside of your cheeks or tongue.

While holding the swab, unscrew the top of the tube and place the swab into the liquid in the tube so that the score ( black) line is at the top of the tube.

Carefully break off the swab at the score line by pressing the shaft of the swab against the side of the tube and discard the top part of the swab shaft.

Screw the cap back onto the tube and ensure it is tightly fastened

Do not pierce the foil lid.

Ensure your name and date of birth is on the tube, hand back to the nurse.

Urine collection

The patient should not have urinated for at least 1 hour prior to collection

Collect the sample into a sterile preservative free collection cup ( this should be the first 20-60 ml of the urine NOT midstream)

Remove the cap from the tube and transfer 2ml of the urine using the disposable pipette provided, ensure the tube is filled to a level between the 2 black collection lines,  label with patient information.

Do not pierce the foil lid.

 

Specimen storage and Transport

After collection, the sample should be stored between 2C and 30C until tested.

Samples should be assayed within 30 days of collection, if longer storage is needed samples may be frozen at -20 to -70 for up to 12 months.

Urine samples NOT in the Aptima tubes ( i.e in primary universals) must be transported to the lab  between 2 and 30C

Urines should be transferred to the Aptima tube within 24 hours of collection.